AAVnerGene is at the forefront of providing innovative solutions for GPCR research. G-protein-coupled receptors (GPCRs) are a large family of receptors that play critical roles in numerous physiological processes and are one of the most sought-after targets in drug discovery. Our AAV ACTOne GPCR Assay Kits offer a cutting-edge platform for high-throughput GPCR screening, functional assays, and drug discovery.

What Are GPCRs?

GPCRs are transmembrane receptors that mediate cellular responses to hormones, neurotransmitters, and environmental stimulants. Their involvement in a wide range of biological processes makes GPCRs key therapeutic targets in areas such as:

• Cardiovascular diseases
• Neurological disorders
• Endocrine diseases
• Immune responses
• Cancer therapy

Given their critical role in human health, precise and efficient GPCR screening methods are crucial for developing new therapeutics.

AAV ACTOne GPCR Assay Kits: A Powerful Tool for GPCR Research

Our AAV ACTOne GPCR Assay Kits offer an advanced, cell-based platform for studying GPCR function in real-time. These kits utilize a cyclic AMP (cAMP) biosensor coupled with a modified cyclic nucleotide-gated (CNG) ion channel that is expressed in the presence of AAV vectors, allowing researchers to monitor GPCR activity in living cells.

Key Features of AAV ACTOne GPCR Kits:

1. High-Throughput Screening: Optimized for large-scale screening of potential GPCR-targeting compounds, enabling fast and accurate identification of leads.
2. Real-Time Detection: Allows for the dynamic monitoring of cAMP levels in live cells, providing real-time insights into GPCR activity.
3. Multiple GPCR Targets: Our kits are compatible with both Gs-GPCRs (stimulatory GPCRs) and Gi-GPCRs (inhibitory GPCRs), giving you the flexibility to study various GPCR pathways.
4. Customizable Options: Tailored for specific GPCRs or cell types, allowing you to design experiments that best suit your research goals.
5. Versatile Applications: Suitable for drug screening, functional assays, signal transduction studies, and more.

How AAV ACTOne GPCR Kits Work:

The AAV ACTOne GPCR Assay Kit combines the power of AAV vectors to deliver the GPCR and CNG channel into cells. When a ligand binds to the GPCR, it triggers a cascade that increases or decreases cAMP levels within the cell. The CNG channel responds to these cAMP changes, causing a measurable ion flux, which can be detected using fluorescent ion indicators or membrane potential dyes.

This system provides a robust and reliable method for:

• Measuring GPCR-mediated cAMP production
• Quantifying GPCR signaling responses
• Identifying agonists, antagonists, and allosteric modulators