AAV Capsid Titer
AAV capsid titer refers to the total number of viral particles—both full (genome-containing) and empty (genome-lacking)—in an AAV preparation. It provides a critical measure of overall particle count, regardless of whether the particles are functional or carry a transgene.
Capsid titer is typically expressed as viral particles per milliliter (vp/mL) and is essential for:
Quality control in AAV manufacturing
Calculating full-to-empty ratios when paired with genomic titer
Assessing batch consistency and capsid yield
Dosing for immunogenicity studies, since empty particles still contribute to immune load
Price and Turnaround
| QC | Method | Turnaround | Serotypes | Price | Sample Requirement |
| AAV Capsid Titer | ELISA | 3-5 day | AAV1, AAV2, AAV3B, AAV5, AAV6, AAV8, AAV9, AAVrh.10 | $400/sample | Serotypes and buffers; ~20 μl of samples |
Notes:
- AAV capsid titer assays for additional serotypes may also be available. Please contact us to confirm availability.
- Volume discounts are available for multiple samples (more than 5).
AAV References for Capsid Titer
Accurate AAV capsid titer measurement requires well-characterized reference materials to ensure consistency, reliability, and comparability across assays and batches. At AAVnerGene, we provide standardized AAV reference materials, either AAV References (truly-empty) and AAV References (byproduct-empty) capsids specifically designed to support ELISA-based and other analytical assays for capsid quantification.
AAV References (truly-empty)
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AAV References (byproduct-empty)
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Elisa for AAV Capsid Titration
AAV capsid titer, also known as AAV concentration or physical titer, is a critical parameter directly linked to dosage, ensuring the safe and effective performance of AAV-based therapies. ELISA (Enzyme-Linked Immunosorbent Assay) is a widely adopted technique for quantifying the total capsid concentration in AAV preparations. This method utilizes specific antibodies to capture and detect AAV capsids, providing a reliable and cost-effective solution for capsid titration.
ELISA is a popular choice among researchers and manufacturers due to its high sensitivity, reproducibility, and accessibility. While it measures the total capsid concentration and does not differentiate between full (genome-containing) and empty capsids, it remains an essential tool for AAV development and quality control. For a more comprehensive analysis, ELISA can be combined with other methods, such as qPCR (to measure genome copies) or MP, CD-MS or AUC (to distinguish full and empty capsids), offering a complete picture of AAV particle composition.
AAV Intact Particle Antibodies
- A20 specifically reacts with intact AAV particles, empty and full capsids
- Analysis of intact AAV particles, i.e. empty and full capsids
- Detection of conformational epitope of the assembled capsid of certain serotypes
- Applications: AAV ELISAs, IF/ICC, affinity chromatography, dot blot, IP, neutralization assay
AAV Intact Particle Antibodies
A69: reacts with VP1/VP2, but not VP3; B1: reacts with VP1, VP2 and VP3; A1: reacts only with VP1
- Analysis of denatured AAV capsid proteins
- Detection of linear epitopes of the viral capsids VP1, VP2 and VP3 across serotypes
Applications: WB, IF/ICC, affinity chromatography, Dotblot, IP
Cross Reactivity & Neutralization
| Antibody Clone | ADK1a | A20 | A20R | ADK4 | ADK5a | ADK5b | ADK6 | ADK8 | ADK8/9 | ADK9 | ADK9-1R | ||||||||||||
| AAV1 | ✓ | ||||||||||||||||||||||
| AAV2 | ✓ | ✓ | |||||||||||||||||||||
| AAV3 | ✓ | ✓ | ✓ | ||||||||||||||||||||
| AAV4 | ✓ | ||||||||||||||||||||||
| AAV5 | ✓ | ✓ | |||||||||||||||||||||
| AAV6 | ✓ | ✓ | |||||||||||||||||||||
| AAV7 | na | ✓ | na | na | |||||||||||||||||||
| AAV8 | ✓ | ✓ | |||||||||||||||||||||
| AAV9 | (✓) | ✓ | ✓ | ||||||||||||||||||||
| AAVrh10 | (✓) | ✓ | |||||||||||||||||||||
| AAV11 | na | na | na | ||||||||||||||||||||
| AAV12 | ✓ | na | na | na | |||||||||||||||||||
| AAVrh74 | na | na | na | na | na | na | ✓ | ✓ | na | na | |||||||||||||
| Anc80 | na | na | ✓ | na | na | na | na | ✓ | ✓ | na | na | ||||||||||||
| AAVDJ | (✓) | na | na | ||||||||||||||||||||
| AAV2.7m8 | na | ✓ | ✓ | na | na | na | na | na | na | na | na |
✓ – Reactivity; (✓) – Weak Reactivity; na – Not analysed: Empty field – no reactivity
Data from: https://us.progen.com/AAV/AAV-Antibodies/AAV-Particle-Antibodies/