AAVdual System

The AAVdual® System is one of  dual-plasmid AAV production systems, which was developed based on our novel mini-pHelper-1.0 plasmid. In this system, the AAV genome carrying the transgene is cloned into the mini-pHelper-1.0, resulting in pAAVdual plasmids. AAV vectors are then generated by co-transfecting pAAVdual plasmids with a standard AAV helper (pRCap) plasmid. This system provides high flexibility and enhances AAV packaging efficiency in vector production.

AAV Production Systems, AAVone, AAVdual and AAVtri

The AAVdual system streamlines the AAV production process, offering several key advantages:

  • Streamline the AAV production process. Compared with traditional triple plasmid system, it reduced plasmid number from 3 to 2, by combining the mini-pHelper with pAAVtri-GOI:
        • pAAVdual Plasmid: Contains the gene of interest (GOI) flanked by inverted terminal repeats (ITRs) for AAV replication and packaging as well as Ad helper genes (E2A, E4orf6 and VA RNA).
        • pRCap: AAV Rep-Cap Plasmid encodes the necessary proteins for AAV genome replication and capsid assembly.
  • High Packaging Efficiency: Demonstrating a significant improvement of 100% to 250% across multiple serotypes when compared to the AAVtri system.
  • Streamlined Workflow: With fewer plasmids to manage, the AAVdual system simplifies the workflow, saving time and resources during production.
  • Flexibility in Serotype Production: Easily generate AAV vectors with different serotype specificities by selecting appropriate Cap genes, all within the same in AAVdual system framework.
  • Compact Plasmid Size: The pAAVdual and pAAVdual-sc plasmids measure only 11±2 kb, making them easier to handle and produce without significantly affecting plasmid yield. This compact size also facilitates cloning procedures.

pAAVdual plasmids:

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Notes:

  • We recommend to use pAAVdual:pRCap at 2:1 molecular ratio for transfection.
  • AAVnerGene also provides DNA synthesis and clone services and different templates (pAAVdual and pAAVdual-sc) for customers to clone their transgenes into our backbone.
  • All the plasmids are kanamycin resistance (KanR). 
  • A Material Transfer Agreement (MTA) is required for academic use of all mini-pHelper–based plasmids, including pAAVdual plasmids, and a license agreement is required for industrial use.
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pRCap plasmids

The pRCap plasmids from AAVnerGene provide essential AAV Rep and Cap functions, enabling robust and reliable AAV vector packaging. Designed for optimal performance, our pRCap plasmids—when used with at AAVdual or AAVtri system—deliver significantly improved packaging efficiency compared to conventional Rep-Cap plasmids.

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Notes:

  • We recommend to use pRCap : pAAVdual at 1:2 molecular ratio for transfection.
  • All the plasmids are ampicillin resistance (Amp). 
  • A Material Transfer Agreement (MTA) is required for academic use of pRCap plasmids, and a license agreement is required for industrial use.
  • AAVnerGene has hundreds of pRCap plasmids with different AAV Caps.

AAVdual-ORF system

This AAVdual-ORF system is designed to use our AAVdual two-plasmid AAV production system to efficiently produce any AAV vectors. Take advantage of our innovative AAVdual system and the comprehensive ORFoeme collections, which contains complete human or mouse ORFs. Researchers only need to provide us the information of gene of interest (GOI) and promoter. After a straightforward one-step reaction, the final AAVdual packaging plasmid carrying the GOI will be constructed for AAV production with AAVdual system, eliminating complex multi-step procedures. The AAVdual-ORF system provides an efficient, one-step solution to advance your AAV research projects more quickly and effectively.

pAAVdual-Pro-CcdB Plasmids:

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