By utilizing different combinations, it is possible to create three distinct types of dual-plasmid systems for AAV production:

• Version V1: Combining Ad helper with GOI
  V1, exemplified by AAVnerGene’s AAVdual system, stands out for its ability to maintain both packaging efficiency and flexibility. This version allows researchers to work with various AAV serotypes while ensuring efficient AAV vector packaging. This balance is particularly beneficial for projects involving diverse gene therapy or vector biology applications.
• Version V2: Combining Ad helper with AAV helper
  V2, such as the pDG and pQT systems, offers flexibility in producing different AAV serotypes, making it useful for a wide range of research applications. However, this version may show lower packaging efficiency due to the complexity of combining Ad and AAV helper plasmids.
• Version V3: Combining GOI with AAV helper
  V3, such as the pOXB system, may provide increased AAV packaging efficiency, which is critical for maximizing the yield of properly packaged AAV vectors. However, it sacrifices flexibility by linking the GOI with the AAV helper plasmid, making it more challenging to switch and customize the GOI.

While all dual-plasmid systems offer specific advantages, only Version V1, as demonstrated by AAVnerGene’s AAVdual system, strikes the optimal balance between packaging efficiency and flexibility.