pAAVone-AAV10-CMV-EGFP

pAAVone-AAV10-CMV-EGFP is a plasmid used to produce AAV10-CMV-EGFP using AAVnerGene’s novel AAVone production system.  It  integrates  all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and AAV10 Cap), and a…

pAAVone-AAV10-CMV-EGFP

$999.00$1,899.00

pAAVone-AAV10-CMV-EGFP is a plasmid used to produce AAV10-CMV-EGFP using AAVnerGene’s novel AAVone production system.  It  integrates  all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and AAV10 Cap), and a single strand AAV-CMV-EGFP genome.   AAV10-CMV-EGFP viruses can be simply and efficiently produced by transfection of this single plasmid into host cells, such us HEK 293 and HEK 293T.
Bacterial Resistance(s) : Kana
Plasmid Backbone: pAAVone
AAV Genome: AAV-CMV-SV40Intron-EGFP-WPRE3-SV40PolyA, Single strand AAV, 2.2 kb
AAV Capsid: AAV10
Storage:  -20°C for long term (> 1 year)
Shipping: Dry ice.
Academic/Nonprofit Terms:  MTA is required.
Industry Terms: Please Contact us to discuss the potential Research Agreement.
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SKU: PB1C10P01Ta01A Categories: , ,

Description

pAAVone-CMV-EGFP Series are plasmids used to produce AAV-CMV-EGFP with different AAV serotypes using AAVnerGene’s novel AAVone production system.  They integrates  all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and Cap), and a single strand AAV-CMV-EGFP genome.   To produce AAV-CMV-EGFP with a certain  serotype, users need select the pAAVone plasmid carrying the corresponding capsid.
Premade serotypes include AAV1, AAV2, AAV2retro AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11, AAV12, AAV13, AAVDJ, AAVrh.10, and AAVrh74. For any other AAV serotypes or variants, please contact us and we will produce them within 2 weeks.

pAAVone-CMV-EGFP Series Plasmids:

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pAAVone-AAV-PHP.eB-CMV-EGFP$999.00$1,899.00
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pAAVone-AAV1-CMV-EGFP$999.00$1,899.00
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pAAVone-AAV10-CMV-EGFP$999.00$1,899.00
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pAAVone-AAV11-CMV-EGFP$999.00$1,899.00
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pAAVone-AAV12-CMV-EGFP$999.00$1,899.00
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pAAVone-AAV13-CMV-EGFP$999.00$1,899.00
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pAAVone-AAV2-CMV-EGFP$999.00$1,899.00
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pAAVone-AAV2-Retro-CMV-EGFP$999.00$1,899.00
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pAAVone-AAV3B-CMV-EGFP$999.00$1,899.00
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pAAVone-AAV4-CMV-EGFP$999.00$1,899.00
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pAAVone-AAV5-CMV-EGFP$999.00$1,899.00
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pAAVone-AAV6-CMV-EGFP$999.00$1,899.00
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pAAVone-AAV7-CMV-EGFP$999.00$1,899.00
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pAAVone-AAV8-CMV-EGFP$999.00$1,899.00
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pAAVone-AAV9-CMV-EGFP$999.00$1,899.00
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pAAVone-AAVrh.10-CMV-EGFP$999.00$1,899.00
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pAAVone-AAVrh74-CMV-EGFP$999.00$1,899.00
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pAAVone-sc-CMV-EGFP Series Plasmids:

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pAAVone-sc-CMV-EGFP Series$999.00$1,899.00
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pAAVone-CMV-mCherry Series Plasmids:

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pAAVone-CMV-mCherry Series$999.00$1,899.00
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Advantages of AAVone system compared with traditional triple plasmid system:

1. Increase AAV yield up to 400%.
2. Reduce plasmid number from 3 to 1.
3.Reduce plasmid amount from 1 to 0.25 ug/1e6 cells.
4.Reduce batch variations.
5.Without need the plasmid ratio optimization steps.
6.Compact plasmid size(16±2kb) and comparable yields.
7.Comparable or increased full-to-empty ratio.
8.Similar viral activity, capsid and genomic components.
9.Similar levels of rcAAV, hcDNAs and producer plasmid contaminations.
10.The same upstream and downstream processes, analytical release or characterization assays.

In summary,  pAAVone-CMV-EGFP series plasmids  offer a simple, low cost, but high consistency and high efficiency for production of AAV-CMV-EGFP virus with different AAV serotypes, especially for large scale and GMP grade AAV production.

 

Workflow of AAVone production system:

Workflow of AAVone System-3

AAVone system increase AAV yield up to 400% compared with traditional triple plasmid system:

AAVone system generated high quality AAV vectors:

 

Additional information

Plasmid Amount

1 mg, 10 mg, 10 ug, 100 ug

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