pAAVdual-hSyn-Transgene Series

pAAVdual-Syn-Transgene Series plasmids are used to produce AAV  viruses with hSyn promoter using AAVnerGene’s novel AAVdual System. In this series plasmids,  the  AAV genomes  with a hSyn promoter  and different transgenes are cloned into our novel Ad helper plasmid, mini-pHelper. …

pAAVdual-hSyn-Transgene Series

$999.00$1,899.00

pAAVdual-Syn-Transgene Series plasmids are used to produce AAV  viruses with hSyn promoter using AAVnerGene’s novel AAVdual System. In this series plasmids,  the  AAV genomes  with a hSyn promoter  and different transgenes are cloned into our novel Ad helper plasmid, mini-pHelper.  Single strand AAV viruses can be generated by co-transfection of pAAVdual plasmids  with the regular AAV helper plasmids, without add additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The Syn promoter, short for Synapsin promoter, is a promoter commonly used in neuroscience research to drive gene expression specifically in neurons, particularly in their synaptic terminals. Synapsins are a family of proteins associated with synaptic vesicles in neurons, and the Syn promoter targets gene expression to these neurons and their synapses.
Bacterial Resistance(s) : Kana
Plasmid Backbone: pAAVdual
AAV Genome Type: Single strand AAV
Promoter: hSyn
Storage:  -20°C for long term (> 1 year)
Shipping: Dry ice.
Academic/Nonprofit Terms:  MTA is required.
Industry Terms: Please Contact us to discuss the potential Research Agreement.
Add To Quote
SKU: N/A Categories: ,

Description

pAAVdual plasmids are used to produce AAV  viruses using AAVnerGene’s novel AAVdual System. In this series plasmids,  the  AAV genomes are cloned into our novel Ad helper plasmid, mini-pHelper.  AAV viruses can be generated by co-transfection of pAAVdual plasmids  with the regular AAV helper plasmids, without add additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.

pAAVdual plasmids with other promoters are available at AAVnerGene:

ImageNamePriceBuy
pAAVdual-CAG-Transgene Series$999.00$1,899.00
Add To Quote
pAAVdual-CAMKII-Transgene Series$999.00$1,899.00
Add To Quote
pAAVdual-CMV-Transgene Series$999.00$1,899.00
Add To Quote
pAAVdual-EF1a-Transgene Series$999.00$1,899.00
Add To Quote
pAAVdual-GFAP-Transgene Series$999.00$1,899.00
Add To Quote
pAAVdual-hSyn-Transgene Series$999.00$1,899.00
Add To Quote
pAAVdual-NSE-Transgene Series$999.00$1,899.00
Add To Quote
pAAVdual-sc-CMV-Transgene Series$999.00$1,899.00
Add To Quote
pAAVdual-sc-hSyn-Transgene Series$999.00$1,899.00
Add To Quote

Advantages of AAVnerGene’s AAVdual system compared with traditional triple plasmid system:

1.Increase AAV yield up to 250%.
2.Reduce plasmid number from 3 to 2.
3.Reduce batch variations.
4.Simply the plasmid ratio optimization processes.
5.Compact plasmid size(11±2kb) and comparable plasmid yields.
6.Comparable or increased full-to-empty ratio.
7.Similar viral activity, capsid and genomic components.
8.Similar levels of rcAAV, hcDNAs and producer plasmid contaminations.
9.The same upstream and downstream processes, analytical release or characterization assays.

This plasmid  offers a relative flexible, simple, low cost, but high consistency and high efficiency for production of AAV virus with different AAV serotypes.

Workflow of AAVdual Production System:

Workflow of AAVone System-3

AAVdual System vs Triple Plasmid System:

Dual plasmid system       

AAVdual System                           Triple Plasmid System

Additional information

Transgene

, , , , , ,

Plasmid Amount

1 mg, 10 mg, 10 ug, 100 ug

Please Login To Download Attachment