pAAVdual plasmids are used to produce AAV viruses using AAVnerGene’s novel AAVdual System. In this series plasmids, the AAV genomes are cloned into our novel Ad helper plasmid, mini-pHelper. AAV viruses can be generated by co-transfection of pAAVdual plasmids with the regular AAV helper plasmids, without add additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
pAAVdual and pAAVdual-sc plasmids with other promoters are available at AAVnerGene:
|pAAVdual-CAG-Transgene Series||$999.00 – $1,899.00|
|pAAVdual-CAMKII-Transgene Series||$999.00 – $1,899.00|
|pAAVdual-CMV-Transgene Series||$999.00 – $1,899.00|
|pAAVdual-EF1a-Transgene Series||$999.00 – $1,899.00|
|pAAVdual-GFAP-Transgene Series||$999.00 – $1,899.00|
|pAAVdual-hSyn-Transgene Series||$999.00 – $1,899.00|
|pAAVdual-NSE-Transgene Series||$999.00 – $1,899.00|
|pAAVdual-sc-CMV-Transgene Series||$999.00 – $1,899.00|
|pAAVdual-sc-hSyn-Transgene Series||$999.00 – $1,899.00|
Advantages of AAVnerGene’s AAVdual system compared with traditional triple plasmid system:
1.Increase AAV yield up to 250%.
2.Reduce plasmid number from 3 to 2.
3.Reduce batch variations.
4.Simply the plasmid ratio optimization processes.
5.Compact plasmid size(11±2kb) and comparable plasmid yields.
6.Comparable or increased full-to-empty ratio.
7.Similar viral activity, capsid and genomic components.
8.Similar levels of rcAAV, hcDNAs and producer plasmid contaminations.
9.The same upstream and downstream processes, analytical release or characterization assays.
This plasmid offers a relative flexible, simple, low cost, but high consistency and high efficiency for production of AAV virus with different AAV serotypes.
Workflow of AAVdual Production System:
AAVdual System vs Triple Plasmid System:
Dual Plasmid System Triple Plasmid System