AAV Serotype Testing Kit-EF1a-mCherry

The AAV Serotype Testing Kit, featuring the EF1α-mCherry system, enables a systematic evaluation of 16 distinct AAV serotypes for gene delivery across diverse cell types and tissues. In this kit, you’ll find a collection of 16 AAV serotypes, each possessing…

AAV Serotype Testing Kit-EF1a-mCherry

$1,199.00

The AAV Serotype Testing Kit, featuring the EF1α-mCherry system, enables a systematic evaluation of 16 distinct AAV serotypes for gene delivery across diverse cell types and tissues. In this kit, you’ll find a collection of 16 AAV serotypes, each possessing unique capsid proteins. These serotypes exhibit variations in their capacity to transduce various cell types and tissues, rendering them valuable tools for refining gene therapy and delivery experiments.

The EF1α promoter serves as the driving force behind the expression of the mCherry reporter gene within these AAV vectors. EF1α is deliberately selected due to its robust and constitutive activity, ensuring consistent gene expression across a wide spectrum of cell types and environmental conditions. This attribute makes it well-suited for evaluating the transduction efficiency of different AAV serotypes within a broad array of mammalian cell types.

By employing mCherry as a reporter, researchers gain the ability to effortlessly visualize and quantify the transduction efficiency of AAV vectors within target cells, further enhancing the precision of their experiments.

  • AAV Expression Cassette: AAV-EF1a-mCherry-WPRE-bGHpolyA.
  • List of 16 Common AAV serotypes: AAV1, AAV2, AAV3B, AAV4-Y729F, AAV5, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAV13, AAV-DJ, AAVrh.10, AAVrh74, AAV2-Retro.
  • Kit Package: 16*50 ul, individual vail, each virus has titer of 1X10^13 VG/ml.
  • Production and Purification: Individual AAV was produced in HEK 293T and purification by two rounds of CsCl.
  • Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl.
  • Storage:  -80°C for long term (> 1 year);  -20°C for short term(1-2 months);  4 °C for 1-2 weeks.
  • Shipping: Dry ice.

50 in stock

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SKU: PK0007 Category:

Description

In this category, 16 most common serotypes of AAV vectors (AAV1, AAV2, AAV3B, AAV4-Y729F, AAV5, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAV13, AAV-DJ, AAVrh.10, AAVrh74, AAV2-Retro) are included in each kit. The vectors carry fluorescent protein (EGFP, mCherry, Tdtomato) or reporter luciferase, which is driven by promoter CAG, CMV, EF1a or hSyn. The kits are pretended to provide useful tools for customers, with minimum cost, in testing AAV transduction efficiency, testing tissue specific tropism related to serotypes, and using as negative control to examine whether the observed biological effects come from specific transgenes. AAV serotype testing kits can be purchased directly as they are, or they can be customized based on the specific needs of the customer.

These kits are designed to quickly and efficiently identify a limited set of commonly used AAV serotypes that are suitable for specific cell lines or tissues.  This method is likely useful when you have a clear understanding of the cell lines or tissues you intend to target and want a simple way to narrow down your choices to a smaller subset of AAV serotypes. The testing process probably involves exposing the cells or tissues to various AAV serotypes and observing how effectively they transduce the target cells. This can provide you with a preliminary indication of which AAV serotypes are most effective for your specific experimental setup.

 

AAV Serotype Testing Kits with different promoters and reporters:

ImageNamePriceBuy
AAV Serotype Testing Kit-CAG-EGFP$1,199.00
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AAV Serotype Testing Kit-CAG-mCherry$1,199.00
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AAV Serotype Testing Kit-CAG-TdTomato$1,199.00
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AAV Serotype Testing Kit-CMV-Fluc$1,199.00
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AAV Serotype Testing Kit-CMV-mCherry$1,199.00
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AAV Serotype Testing Kit-EF1a-EGFP$1,199.00
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AAV Serotype Testing Kit-EF1a-mCherry$1,199.00
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AAV Serotype Testing Kit-hSyn-mCherry$1,199.00
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AAVnerGene offers a versatile range of custom AAV testing kits tailored to meet the specific requirements of their customers. These kits can be customized not only in terms of the reporter gene but also with the choice of promoter, allowing researchers to fine-tune their experiments according to their unique needs. One standout feature of AAVnerGene is its extensive library of over 1000 different capsids readily available for inclusion in these custom kits. This diversity of capsids provides researchers with a wide selection of AAV vectors, each with potentially distinct properties, which can be invaluable for optimizing and tailoring gene delivery approaches for various applications.

Additional information

Promoter

Serotype

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Transgene

Titer (GC/ml)

1.00E+13

Size

16x50ul

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