AAV-EF1a-TdTomato

AAV-EF1a-TdTomato series viruses are ready to use AAV vectors carrying EF1a-TdTomato expression cassette. EF1α is deliberately selected due to its robust and constitutive activity, ensuring consistent gene expression across a wide spectrum of cell types and environmental conditions. This attribute…

AAV-EF1a-TdTomato

$299.00$69,999.00

AAV-EF1a-TdTomato series viruses are ready to use AAV vectors carrying EF1a-TdTomato expression cassette.
EF1α is deliberately selected due to its robust and constitutive activity, ensuring consistent gene expression across a wide spectrum of cell types and environmental conditions. This attribute makes it well-suited for evaluating the transduction efficiency of different AAV serotypes within a broad array of mammalian cell types.
By employing TdTomato as a reporter, researchers gain the ability to effortlessly visualize and quantify the transduction efficiency of AAV vectors within target cells, further enhancing the precision of their experiments.

AAVnerGene offers >50  premade AAV-EF1a-TdTomato vectors with different capsids and have >1000 AAV serotypes/variants to be choose for custom AAV packaging.

  • AAV Expression Cassette: AAV-EF1a-TdTomato.
  • AAV Titer: > 1X10^13 VG/ml.
  • Production and Purification: Individual AAV was produced in HEK 293T and purified by two rounds of CsCl.
  • Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl.
  • Storage:  -80°C for long term (> 1 year);  -20°C for short term(1-2 months);  4 °C for 1-2 weeks.
  • Shipping: Dry ice.

 

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Description

Premade AAV vectors  are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation.  The premade AAV vectors in AAVnerGene are available in various serotypespromoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. Over a thousand of known serotypes of AAV vectors provide customer tools for quickly determination of the AAV vector specificity for different types of cells, while multiple promoters with reporter genes make it easy to determine and tracking the level and timing of a specific gene expression.

To ensure high-quality and pure products, AAVnerGene uses various assays to assess the quality and purity of their pre-made AAV vectors. These assays include qPCR titer targeting the transgenes to determine the concentration of AAV particles carrying the desired transgene, endotoxin testing to determine the level of bacterial contamination, SDS-PAGE gel to determine the purity of the AAV particles, and infectivity assays to determine the efficiency of the AAV particles in transducing target cells. These high purity and high quality of premade AAV vectors provide customer advantages such as less optimization, less labor and expertise required, and helps to ensure reliable and consistent results. They can be used for both in vitro and in vivo applications.

AAVnerGene offers a variety of pre-made AAV vectors with combination of different genome types(ssAAV or scAAV), serotypes(over 1000), promoters (CAG, CMV, EF1a, hSYN, CBh, mPGK, TBG, CaMKIIa, etc), fluorescent proteins (EGFP, mCherry, TdTomato) and reporters (Gluc, Cluc, Fluc, Rluc). The vectors can be purchased individually, or as part of a testing kit. AAVnerGene also provide custom AAV design and package services and can produce any known serotypes or variants.

 

Premade AAV vectors with EF1a promoter:

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AAV-EF1a-Cre$299.00$69,999.00
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AAV-EF1a-EGFP$299.00$69,999.00
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AAV-EF1a-Fluc$299.00$69,999.00
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AAV-EF1a-mCherry$299.00$69,999.00
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AAV-EF1a-TdTomato$299.00$69,999.00
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Premade AAV vectors with TdTomato transgene:

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AAV-CAG-TdTomato$299.00$69,999.00
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AAV-CaMKII-TdTomato$299.00$16,999.00
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AAV-CMV-TdTomato$299.00$69,999.00
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AAV-EF1a-TdTomato$299.00$69,999.00
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AAV-GFAP-TdTomato$299.00$69,999.00
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AAV-hSyn-TdTomato$299.00$69,999.00
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AAV-NSE-TdTomato$299.00$69,999.00
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Additional information

Serotype

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Viral Amount

1E12 VG, 1E13 VG, 1E14 VG, 1E15 VG, 2E12 VG, 2E13 VG, 2E14 VG, 5E12 VG, 5E13 VG, 5E14 VG

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