Tissue-specific ATHENA I AAV capsid libraries
Tissue-specific ATHENA I AAV capsid libraries are designed to evaluate AAV capsid variants that have been engineered to exhibit enhanced tropism for specific target tissues. It also includes 16 commonly used AAV serotypes that serve as controls, which are essential for benchmarking and comparative purposes.
|AAV Capsid Library(I)-BBB||AAV-B1, AAV-F, AAV-MaCPNS1, AAV-PHP.B, AAV-PHP.C2, AAV-PHP.eB, AAV-PHP.S, AAV.Cap-B22, AAV/Olig001.||$9,999.00 – $54,999.00||aav-b1 aav-f aav-macpns1 aav-php-b aav-php-c2 aav-php-eb aav-php-s aav-cap-b22 aav-olig001|
|AAV Capsid Library(I)-Common||No additional tissue-targeting capsids||$5,999.00 – $32,999.00||no-additional-tissue-targeting-capsids|
|AAV Capsid Library(I)-Lung||AAV2-ESGHGYF, AAV2-PRSTSDP, AAV2-Y444F-Y500F, AAV2.5T, AAV2H22, AAV5-PK2, AAV6.2, AAV6.2FF, AAV9.452sub.LUNG1||$9,999.00 – $54,999.00||aav2-esghgyf aav2-prstsdp aav2-y444f-y500f aav2-5t aav2h22 aav5-pk2 aav6-2 aav6-2ff aav9-452sub-lung1|
Design of AAV Capsid Library(I):
In the AAV capsid libraries(I), each capsid variant is associated with three different DNA-barcoded genomes, and all three of these genomes carry the same reporter gene. The use of three DNA barcodes for one capsid variant can minimize experimental variations and improve the accuracy of the results in high-throughput screening or selection processes.
In the standard AAV capsid libraries(I), AAV genomes carried a CAG promoter drive EGFP expression, following by the Woodchuck hepatitis virus post-transcriptional regulatory element(WPRE) enhancer and a bovine growth hormone polyadenylation signal(bGH polyA). The unique barcode is located between the coding sequence and the Poly-A signal. The CAG promoter is known for strong and ubiquitous expression in various cell types. The presence of the EGFP reporter gene allows for the enrichment of transgene-expressing cells, making it possible to identify and isolate cells that have taken up and expressed the AAV genomes. WPRE enhancer and bGH polyA can enhance EGFP expression and stabilize mRNA.
Researchers can use the unique DNA barcodes to evaluate the distribution of each capsid variant for different types of target cells. This screening method helps identify which AAV capsids are most effective at transducing specific cell types. In addition to DNA barcode screening, transcribed RNA barcodes can be evaluated to assess the potential of different capsids for each target cell. This approach provides insights into the transcriptional activity of the AAV genomes in the context of specific cell types.
NGS technology is used to analyze the barcode data. It allows researchers to simultaneously assess hundreds of AAV vectors at once, both in vivo (in living organisms) and in vitro (in cell culture).
Common AAV Capsid List: AAV1, AAV2, AAV3B, AAV4-Y729F, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAV11, AAV12, AAV13, AAVrh74, AAV-DJ, AAV2-Retro